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Image Search Results
Journal: Oncotarget
Article Title: In-depth proteomic profiling of the uveal melanoma secretome
doi: 10.18632/oncotarget.10418
Figure Lengend Snippet: Brightfield images of UM cells with a spindle phenotype ( A and B ) and plump epithelioid-like cells ( C ). Immunofluorescence phenotyping of UM ( D, E and F ) and NCM ( G, H and I ) cells in culture. UM cells expressing (D) MelanA, (E) HMB45 and (F) vimentin. Positive staining was detected with Alexa 488 (green) and nuclei were counterstained with DAPI (blue). NCM expressing (G) MelanA, (H) gp100 and (I) HMB45. Positive staining was detected with Alexa 488 (green) and nuclei were counterstained with propidium iodide (red).
Article Snippet: Immunofluorescence staining was performed as previously described [ ], using the following antibodies for 30min at room temperature (RT);
Techniques: Immunofluorescence, Expressing, Staining